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浅谈新冠病毒的核酸检测法


  • 浅谈新冠病毒的核酸检测法
    1. 浅谈新冠病毒的核酸检测法

浅谈新冠病毒的核酸检测法

新型冠状病毒的检测方法主要包含核酸检测、抗体检测、抗原检测几种方法。由于抗原检测检出率较低,目前新冠检测主要集中在抗体和核酸检测。核酸检测目前是新型冠状病毒检测的“金标准”,具有早期诊断、灵敏度和特异

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信息详情

XINXINGGUANZHUANGBINGDUDEJIANCEFANGFAZHUYAOBAOHANHESUANJIANCE、KANGTIJIANCE、KANGYUANJIANCEJIZHONGFANGFA。YOUYUKANGYUANJIANCEJIANCHULVJIAODI,MUQIANXINGUANJIANCEZHUYAOJIZHONGZAIKANGTIHEHESUANJIANCE。HESUANJIANCEMUQIANSHIXINXINGGUANZHUANGBINGDUJIANCEDE“JINBIAOZHUN”,JUYOUZAOQIZHENDUAN、LINGMINDUHETEYIXINGGAODENGTEDIAN;DANKANGTIJIANCECAOZUOBIANJIE、JIANCEXUNSU,KEZUOWEIHESUANZHENDUANDEBUCHONGSHOUDUAN,DANYOUYUKANGTIJIANCE“JIAYANGXING”HE“JIAYINXING”DEJUXIANXING,BUSHIHEYONGYUFUGONGFUCHANFUXUEDENGPUTONGRENQUNSHAICHA,YEBUSHIYONGYUZAIDILIUXINGDIQUDELIUXINGBINGXUEDIAOCHA。

JIANCEYUANLI:

SUOYOUSHENGWUCHURUANBINGDUWAIDOUHANYOUHESUAN,HESUANBAOKUOTUOYANGHETANGHESUAN(DNA)HEHETANGHESUAN(RNA)。XINXINGGUANZHUANGBINGDU(2019-nCoV)SHUYUBetaGUANZHUANGBINGDUSHU(Betacoronavirus),GAIBINGDUSHIDANBAIBAOGUODEDANLIANZHENGLIANRNABINGDU,JISHENGHEGANRANGAODENGDONGWU(BAOKUOREN)。BINGDUZHONGTEYIXINGRNAXULIESHIQUFENGAIBINGDUYUQITABINGYUANTIDEBIAOZHIWU,XINXINGGUANZHUANGBINGDUCHUXIANHOU,WOGUOKEXUEJIAYIJINGCHENGGONGFAXIANLEXINXINGGUANZHUANGBINGDUZHONGDETEYIXINGHESUANXULIE。RUYISIHUANZHEYANGBENZHONGNENGJIANCEDAOXINXINGGUANZHUANGBINGDUDETEYIXINGHESUANXULIE,ZERENWEIGAIHUANZHEKENENGBEIXINXINGGUANZHUANGBINGDUGANRAN。 XINXINGGUANZHUANGBINGDUCHANGYONGDEHESUANZHENDUANFANGFAYOULIANGZHONG:BINGDUHESUANTEYIJIYINJIANCEHEBINGDUJIYINZUCEXU。ZUICHANGJIANDEJIANCEXINXINGGUANZHUANGBINGDUTEYIXINGHESUANXULIEDEFANGFASHIYINGGUANGDINGLIANGPCR(JUHEMEILIANSHIFANYING)。YOUYUXINXINGGUANZHUANGBINGDUSHIRNABINGDU,SHIJIHEJIANCEJIBENDOUCAIYONGFANZHUANLUJIASHISHIJUHEMEILIANSHIFANYINGFA(RT-PCR),KUOZENGBINGYUANTIDEHESUAN (RNA) ,TONGSHITONGGUOYINGGUANGTANZHENSHISHIJIANCEKUOZENGCHANWU。ZAIPCRFANYINGTIXIZHONG,BAOHANYIDUITEYIXINGYINWUYIJIYIGETaqmanTANZHEN,GAITANZHENWEIYIDUANTEYIXINGGUAHEGANSUANXULIE,LIANGDUANFENBIEBIAOJILEBAOGAOYINGGUANGJITUANHECUIMIEYINGGUANGJITUAN。TANZHENWANZHENGSHI,BAOGAOJITUANFASHEDEYINGGUANGXINHAOBEICUIMIEJITUANXISHOU;RUFANYINGTIXICUNZAIBAXULIE,PCRFANYINGSHITANZHENYUMOBANJIEHE,DNAJUHEMEIYANMOBANLIYONGMEIDEWAIQIEMEIHUOXINGJIANGTANZHENMEIQIEJIANGJIE,BAOGAOJITUANYUCUIMIEJITUANFENLI,FACHUYINGGUANG。MEIKUOZENGYITIAODNALIAN,JIUYOUYIGEYINGGUANGFENZICHANSHENG。YINGGUANGDINGLIANGPCRYINENGGOUJIANCECHUYINGGUANGDAODAYUXIANSHEDINGYUZHIDEXUNHUANSHU(CtZHI)YUBINGDUHESUANNONGDUYOUGUAN,BINGDUHESUANNONGDUYUEGAO,CtZHIYUEXIAO。BUTONGSHENGCHANQIYEDECHANPINHUIYIJUZISHENCHANPINDEXINGNENGQUEDINGBENCHANPINDEYANGXINGPANDUANZHI。

YOUYUMEIGERT-PCRFANYINGXUYAO2GEXIAOSHIZUOYOUCAICHUJIEGUO,YINCI,SHIJIHEZAIKAIFASHIYIBANDOUZHENDUIBINGDUXULIEZHONGGAODUBAOSHOUDE2-3GEXULIEBIRUBIANMAreplicase(FUZHIMEI)HUOZHEnecleocapsid(HEDANBAIYI,HEQIAO)DEHESUANXULIESHEJIYINWU,DANSHIGEGEFANGFAYONGDEXULIEDOUBUWANQUANXIANGTONG。YIXIEFANGFASHIYONGmultiplex,YEJIUSHIBADUOGEMUBIAOXULIEZAITONGYIFANYINGGUANZHONGKUOZENG;LINGYIXIECAIYONGFENBU,XIANYONGYIGEMUBIAOXULIESHAICHA,YANGXINGHOUZAIYONGLINGYIGEXULIEQUEREN,ZHEIYANGKEYIYOUXIAOSUODUANZHENGGELIUCHENGSHIJIAN,JIAKUAIJIANCESUDU。LINGWAIRNABINGDUBIANYIHENKUAI,YONGDUOGEBAOSHOUMUBIAOXULIEKEYIFANGZHIBINGDUBIANYIZAOCHENGDEJIAYINXING。

MUQIANPIZHUNCHANPINJUNJIYUXINXINGGUANZHUANGBINGDUJIYINZUZHONGKAIFANGDUMAKUANG1a/b(open reading frame 1ab,ORF1ab)、BAOMODANBAI(Envelope protein,E)HEHEYIQIAODANBAI(nucleocapsid protein,N)JINXINGXUANZE。BUTONGCHANPINDEJIANCEYUANLIJIBENYIZHI,DANSHIQIYINWU、TANZHENSHEJICUNZAIBUTONG,YOUDANBAQUDUAN(ORF1ab)、SHUANGBAQUDUAN(ORF1ab、NDANBAI)、SANBAQUDUAN(ORF1ab、NDANBAIHEEDANBAI)DEJIANCEHEPANDUCHABIE。YIBANJIANCEWEIYUBINGDUORF1abHENJIYINSHANGDELIANGGEBABIAO,TONGYIFENBIAOBENXUMANZUSHUANGBABIAOYANGXINGHUOZHONGFUJIANCEWEIDANBABIAOYANGXINGHUOLIANGZHONGBIAOBENTONGSHIMANZUDANBABIAOCAINENGQUERENSARS-CoV-2BINGDUHESUANYANGXING。

JIANCELIUCHENG:

检测程序需要经过五个步骤,取样、留样、保存、核酸提取、上机检测。首先根据试剂盒说明书进行样本采集,样本类型包括咽拭子、鼻拭子、痰液、支气管灌洗液、肺泡灌洗液等。由于RNA易降解,因此,采集样本时使用无RNA酶的拭子和无RNA酶的储存管。获得患者样本后,需尽快进行检测,如无法立即检测需要进行低温封装,并送到专门的检测机构进行检测。检测机构收到样本后,对样本进行核酸提取,核酸提取试剂应使用批准产品说明书中指定的核酸提取试剂盒。最后是荧光PCR核酸检测,也就是上机器检测,将提取物进行荧光PCR扩增反应,需要70—80分钟。

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浅谈新冠病毒的核酸检测法 2020-7-22 本文被阅读 277 次
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